细胞培养支原体污染：发生率，来源，效果，检测，消除，预防

细胞培养物由支原体污染保持在细胞培养物中的一个主要问题。支原体能产生无限种在他们感染的培养影响。本文缔一生物/缔一生物为您分析细胞培养支原体污染：发生率，来源，效果，检测，消除，预防。

　　这些微生物是在细胞培养中常用的多数抗生素抗性。在这里，我们提供关于当前知识的一简要的概述：

（1）发生率和支原体污染的在细胞培养物来源，支原体物种在细胞培养物zui常检测到的，和支原体的上的功能和被感染细胞培养物的影响的活动;

（2）可用于检测特别强调zui可靠的检测方法支原体的各种技术;

（3）可用于消除支原体突出抗生素治疗的各种方法;

（4）推荐用于支原体污染的检测，消除和预防程序和工作协议。准确，灵敏和可靠的检测方法的可用性和健壮和成功的消除方法的应用提供用于克服在细胞培养物的支原体污染问题的有力手段。

英文原文：

The contamination of cell cultures by mycoplasmas remains a major problem in cell culture.Mycoplasmas can produce a virtually unlimited variety of effects in the cultures they infect. These organisms are resistant to most antibiotics commonly employed in cell cultures.

Here we provide a concise overview of the current knowledge on:

(1) the incidence and sources of mycoplasma contamination in cell cultures, the mycoplasma species most commonly detected in cell cultures, and the effects of mycoplasmas on the function and activities of infected cell cultures;

(2) the various techniques available for the detection of mycoplasmas with particular emphasis on the most reliable detection methods;

(3) the various methods available for the elimination of mycoplasmas highlighting antibiotic treatment;

(4) the recommended procedures and working protocols for the detection, elimination and prevention of mycoplasma contamination. The availability of accurate, sensitive and reliable detection methods and the application of robust and successful elimination methods provide powerful means for overcoming the problem of mycoplasma contamination in cell cultures.

综上所述，您是不是已经对细胞培养支原体污染：发生率，来源，效果，检测，消除，预防，有所了解。如果还有其他疑问，请咨询缔一生物/缔一生物资深专家：。